ZC3HC1 antibody

Catalog No. ABIN498264

The Rabbit Polyclonal anti-ZC3HC1 antibody (ABIN498264) specifically detects ZC3HC1 in WB and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ZC3HC1 antibody (ABIN498264)

Target: ZC3HC1 (Zinc Finger, C3HC-Type Containing 1 (ZC3HC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZC3HC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ZC3HC1 Antibody

Specificity: This antibody detects endogenous levels of NIPA protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity-Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH ~7.2 with 0.05 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ZC3HC1

Target: ZC3HC1 (Zinc Finger, C3HC-Type Containing 1 (ZC3HC1))

Alternative Name: ZC3HC1

Background: Entry into mitosis is essentially driven by cyclin B1, which is located in the cytoplasm throughout interphase, but accumulates in the nucleus just before mitosis occurs. Nuclear interaction partner of ALK (NIPA) plays a critical role in cyclin B1 regulation. NIPA is normally phosphorylated during G2 and M phases, resulting in an accumulation of cyclin B1. When NIPA sheds its attached phosphate, it binds to SCF to form the SCFNIPA complex, a member of the E3 ubiquitin ligases, which ubiquitinates cyclin B1, thereby targeting it to the proteosome for degradation. Therefore, the accumulation of cyclin B1 is due to the inability of phosphorylated NIPA to bind to the molecule SCF, thereby preventing the degradation of cyclin B1. An absence of NIPA causes cyclin B1 to accumulate abnormally, leading to premature mitotic entry, loss of checkpoint control and genomic instability, which are all associated with cancer. The phosphorylated form of NIPA may also be involved in apoptotic signaling pathways.Synonyms: HSPC216, NIPA, Nuclear-interacting partner of anaplastic lymphoma kinase (ALK), Zinc finger C3HC-type protein 1

Molecular Weight: approx. 55 kDa

Gene ID: 51530

NCBI Accession: NP_057562

UniProt: Q86WB0