CSNK1A1 antibody

Catalog No. ABIN498265

This Rabbit Polyclonal antibody specifically detects CSNK1A1 in WB, IF and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for CSNK1A1 antibody (ABIN498265)

Target: CSNK1A1 (Casein Kinase 1, alpha 1 (CSNK1A1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSNK1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CSNK1A1 Antibody

Specificity: This antibody detects endogenous levels of Casein Kinase I α protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for CSNK1A1

Target: CSNK1A1 (Casein Kinase 1, alpha 1 (CSNK1A1))

Alternative Name: Casein Kinase I alpha

Background: Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Iα, Iγ, Iδ and Iε, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an α2β2 or an αα'β2 structure. The a catalytic subunit is stimulated by the β regulatory subunit, which undergoes autophosphorylation. Casein kinase II activity is high in the cytosol and nucleus of proliferating and differentiating cells. Casein kinase II is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.Synonyms: CK1, CKI-alpha, CSNK1A1

Molecular Weight: approx. 35 kDa

Gene ID: 1452

NCBI Accession: NP_001883

UniProt: P48729

Pathways: WNT Signaling, Hedgehog Signaling