DGKE antibody

Catalog No. ABIN498319

This Rabbit Polyclonal antibody specifically detects DGKE in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DGKE antibody (ABIN498319)

Target: DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGKE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DGKE Antibody

Specificity: This antibody detects endogenous levels of DGK-ε protein. (region surrounding Ser194)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for DGKE

Target: DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))

Alternative Name: DAG Kinase epsilon

Background: Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-α (80 kDa), -β (90 kDa), and -γ (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-δ (130 kDa) and DGK-?(130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-ε (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-ζ (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-θ (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.Synonyms: DAGK5, DGK-epsilon, DGKE, Diacylglycerol kinase epsilon, Diglyceride kinase epsilon

Molecular Weight: approx. 60 kDa

Gene ID: 8526

NCBI Accession: NP_003638

UniProt: P52429