CKS1 antibody

Catalog No. ABIN498326

The Rabbit Polyclonal anti-CKS1 antibody is suitable to detect CKS1 in samples from Human and Mouse. It has been validated for WB and IF.

Quick Overview for CKS1 antibody (ABIN498326)

Target: CKS1 (CKS1B) (CDC28 Protein Kinase Regulatory Subunit 1B (CKS1B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-CKS1 Antibody

Specificity: This antibody detects endogenous levels of Cks1 protein. (region surrounding His3)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CKS1

Target: CKS1 (CKS1B) (CDC28 Protein Kinase Regulatory Subunit 1B (CKS1B))

Alternative Name: CKS1B

Background: The Cdc2 p34-cyclin B complex plays a critical role in the cell cycle by regulating the G2 to M transition. Also referred to as M-phase promoting factor or MPF, this complex is a required component of the cell cycle machinery and is necessary for cell entry into mitosis. The Cdc28 protein represents the S. cerevisiae counterpart of human Cdc2 p34 and has been found complexed to a regulatory protein, termed p13suc 1, in addition to cyclin B. The human homolog of p13suc 1 has been identified and designated Cks1 p9. Null mutations in the p13suc 1 and Cks1 p9 genes result in the arrest of the cell cycle at either the G1 or G2 phase, suggesting that the proteins may also regulate the activity of cyclin dependent kinases that act at critical points early in the cell cycle.Synonyms: CKS-1, CKS1, Cyclin-dependent kinases regulatory subunit 1, PNAS-143, PNAS-16

Molecular Weight: approx. 9 kDa

Gene ID: 1163

NCBI Accession: NP_001817

UniProt: P61024

Pathways: Mitotic G1-G1/S Phases, Inositol Metabolic Process