HES6 antibody

Catalog No. ABIN498352

This Rabbit Polyclonal antibody specifically detects HES6 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for HES6 antibody (ABIN498352)

Target: HES6 (Hairy and Enhancer of Split 6 (HES6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HES6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HES6 Antibody

Specificity: This antibody detects endogenous levels of HES-6 protein. (region surrounding Ala6)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HES6

Target: HES6 (Hairy and Enhancer of Split 6 (HES6))

Alternative Name: HES6

Background: The Drosophila hairy and enhancer of split genes encode basic helix-loophelix (bHLH) transcriptional repressors that function in the notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homologues of Drosophila hairy and enhancer of split are the HES gene family members, HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homologue of groucho, and this interaction is mediated by the carboxy-terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N-box (CACNAG) as opposed to the E-box (CANNTG). HES2 binds to both N- and E-box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues.Synonyms: BHLHB41, C-HAIRY1, Class B basic helix-loop-helix protein 41, Hairy and enhancer of split 6, Transcription cofactor HES-6

Molecular Weight: approx. 30 kDa

Gene ID: 55502

NCBI Accession: NP_061115

UniProt: Q96HZ4