14-3-3 eta antibody

Catalog No. ABIN498365

The Rabbit Polyclonal anti-14-3-3 eta antibody is suitable to detect 14-3-3 eta in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for 14-3-3 eta antibody (ABIN498365)

Target: 14-3-3 eta (YWHAH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 eta antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-14-3-3 eta Antibody

Specificity: This antibody detects endogenous levels of 14-3-3 theta protein. (region surrounding Lys81)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate Buffered Saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for 14-3-3 eta

Target: 14-3-3 eta (YWHAH)

Alternative Name: 14-3-3 Protein eta

Background: 14-3-3 eta (YWHAH) belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine or phosphothreonine-containing proteins. YWHAH is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. It binds to a large number of partners and binding generally results in the modulation of the activity of the binding partner. The YWHAH gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia.Synonyms: Protein AS1, YWHA1, YWHAH

Molecular Weight: approx. 42 kDa

Gene ID: 7533

NCBI Accession: NP_003396

UniProt: Q04917

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, C21-Steroid Hormone Metabolic Process, Myometrial Relaxation and Contraction