HTR1F antibody

Catalog No. ABIN498368

The Rabbit Polyclonal anti-HTR1F antibody is suitable to detect HTR1F in samples from Human, Rat and Mouse. It has been validated for WB and IF.

Quick Overview for HTR1F antibody (ABIN498368)

Target: HTR1F (5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTR1F antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-HTR1F Antibody

Specificity: This antibody detects endogenous levels of SR-1F protein. (region surrounding Thr126)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunofluorscence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HTR1F

Target: HTR1F (5-Hydroxytryptamine (serotonin) Receptor 1F (HTR1F))

Alternative Name: Serotonin Receptor 1F (HTR1F)

Background: The members of the G protein-coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. beta-adrenergic bound to adrenaline activates adenylyl cyclase, while alpha2-adrenergic receptor bound to adrenaline inhibits adenylyl cyclase. Like the alpha2-adrenergic receptor, serotonin receptor functions are also mediated by G proteins that inhibit the activity of adenylyl cyclase. The serotonin receptors have been classified into several categories, designated SR-1-7 (5HT1-7). Subtypes within the SR-1 group include SR-1A, -1B, -1D, -1E and -1F.Synonyms: 5-HT-1F, 5-hydroxytryptamine receptor 1F, HTR1EL

Molecular Weight: approx. 38 kDa

Gene ID: 3355

NCBI Accession: NP_000857

UniProt: P30939

Pathways: JAK-STAT Signaling