»
Antibodies
»
anti-Allograft Inflammatory Factor 1 (AIF1) antibody
Allograft Inflammatory Factor 1 (AIF1) antibody
| Antigen |
|
| Synonyms |
IBA1, AIF-1, IRT-1, G1, Iba1, AI607846, D17H6S50E, iba1, Bart1, mrf-1, fa04b11, wu:fa04b11, AIF1, AIF, aif1 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|
| Catalog no. |
ABIN498378 |
| Quantity |
100 µg (1,0 mg/ml) (Variants) |
| Price |
340.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 7 to 10 Business Days |
|
Characteristics
|
Synonyms: AIF-1, Protein G1, IBA1, Allograft inflammatory factor 1, Ionized Calcium-Binding AdapterMolecule 1
|
|
Alternative name
|
AIF1
|
|
Gene ID
|
199
|
|
Swiss-Prot
|
P55008
|
|
Cross-Reactivity
|
Human, Mouse (Murine), Rat (Rattus)
|
|
Description
|
Apoptosis is characterized by several morphological nuclear changes including chromatincondensation and nuclear fragmentation. These changes are triggered by the activation ofmembers of caspase family, caspase activated DNase, and several novel proteins. A novelgene, the product of which causes chromatin condensation and DNA fragmentation, wasrecently identified, cloned, and designated apoptosis inducing factor (AIF). Like the criticalmolecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIFtranslocates to the nucleus when apoptosis is induced and induces mitochondria torelease the apoptogenic proteins cytochrome c and caspase 9. AIF induces chromatincondensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, ofthe isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.
|
|
Specificity
|
AIF-M1 pAb detects endogenous levels of AIF-M1 protein. Species: Human, Mouse, Rat. Others not tested.
|
|
Application Notes
|
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
|
|
Concentration
|
1,0 mg/ml
|
|
Purification
|
Aff - Purified
|
|
Buffer
|
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
|
|
Storage
|
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
|
|
Research Area
|
Inflammation
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Allograft Inflammatory Factor 1 (AIF1)", type "Antibodies"
|
Hosts
|
Rabbit (19), Goat (8), Mouse (8)
|
|
Reactivities
|
Human (30), Rat (Rattus) (13), Mouse (Murine) (9), Dog (Canine) (3), Cow (Bovine) (2)
|
|
Applications
|
Western Blotting (WB) (29), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Enzyme Immunoassay (EIA) (6), ELISA (5), ELISA (Detection) (3), Immunofluorescence (IF) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunoprecipitation (IP) (2), Flow Cytometry (FACS) (1), Immunohistochemistry (IHC) (1)
|
|
Conjugates
|
Biotin (1), FITC (1)
|
|
Epitopes
|
isoform 1 and 3 (3), isoform 3 (2)
|
