EIF4G2 antibody

Catalog No. ABIN498384

The Rabbit Polyclonal anti-EIF4G2 antibody (ABIN498384) specifically detects EIF4G2 in WB, IF and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for EIF4G2 antibody (ABIN498384)

Target: EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4G2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF4G2 Antibody

Specificity: This antibody detects endogenous levels of DAP-5 protein. (region surrounding asn65)

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohisochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EIF4G2

Target: EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))

Alternative Name: eIF4G2 / DAP5

Background: Death-associated protein 5 (DAP-5) (also known as p97 and NAT1) is a member of the eukaryotic translation initiation factor 4G (elF4G) family. DAP-5 is ubiquitously expressed and is highly conserved among species. In response to activated FAS or p53, caspase cleaves DAP-5 at position 790 to yield a C-terminal truncated protein which is capable of forming complexes with eIF4A and eIF3. DAP-5 has homology to the carboxy-terminal portion of eIF4G, but lacks the N-terminal region of eIF4G, which is responsible for association with the CAP binding protein eIF4E. By forming translationally inactive complexes with eIF4A and eIF3, but not with eIF4E, DAP-5 functions as a general repressor of translation. During apotosis, the caspase-activated DAP-5 can mediate CAP-independent translation at least from its own internal ribosome entry site, thus resulting in a positive feedback loop responsible for the continuous translation of DAP-5. DAP-5 is also required for cellular differentiation, as it controls specific gene expression pathways.Synonyms: AAG1, Death-associated protein 5, Eukaryotic translation initiation factor 4 gamma 2, FLJ41344, NAT1, eIF-4-gamma 2, p97

Molecular Weight: approx. 90 kDa

Gene ID: 1982

NCBI Accession: NP_001409

UniProt: P78344