Laminin, gamma 1 (LAMC1) antibody

Antigen: Laminin, gamma 1 (LAMC1)

Synonyms: LAMB2, MGC87297, B2e, sly, LAMC1, lamb2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN498386

Additional Information

Characteristics: Synonyms: LAMC1, LAMB2, Laminin subunit gamma-1, Laminin B2 chain

Alternative name: Laminin gamma 1

Gene ID: 3915

Swiss-Prot: P11047

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Laminins are essential and abundant structural non-collagenous glycoproteins localizingto basement membranes. Basement membranes (cell-associated extracellular matrices(ECMs)) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen andseveral proteoglycans. Basement membranes are found under epithelial layers, around theendothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration,gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta andgamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules and have unique andshared cell interactions mediated by Integrins, dystroglycan and cognate Lamininreceptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and isubiquitously expressed in tissues that produce basement membranes.

Specificity: This antibody detects endogenous levels of Laminin gamma-1 protein. Species: Human, Mouse and Rat. Others not tested. Add. Information: Molecular Weight: ~178. 0 kda

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM Sodium Azideas preservative, approx. pH 7.2.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Neurology

Restrictions: For Research Use only