NFYC antibody

Antigen: NFYC

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN498393

Additional Information

Characteristics: Synonyms: Nuclear transcription factor Y subunit gamma, Transactivator HSM-1/2

Gene ID: 4802

Swiss-Prot: Q13952

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically bindsto CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA,NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Ysubunit, the segment needed for formation of the NF-Y-DNA complex is conserved fromyeast to human. These conserved segments are homologous to the histone-fold motif ofeukaryotic histones. The DNA binding domains of the NF-YB and NF-YC subunits have beensuggested to interact through a protein-protein histone-fold “handshake” motif in amanner analogous to the histone proteins, H2B and H2A, respectively.

Specificity: NF-YC pAb detects endogenous levels of NF-YC protein. Species: Human, Mouse, Rat. Others not tested.

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1,0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only