NFYC antibody

Catalog No. ABIN498393

The Rabbit Polyclonal anti-NFYC antibody (ABIN498393) specifically detects NFYC in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NFYC antibody (ABIN498393)

Target: NFYC (Nuclear Transcription Factor Y, gamma (NFYC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NFYC Antibody

Specificity: This antibody detects endogenous levels of NF-YC protein. (region surrounding Trp85)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NFYC

Target: NFYC (Nuclear Transcription Factor Y, gamma (NFYC))

Alternative Name: NFYC

Background: The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein histone-fold Synonyms: Nuclear transcription factor Y subunit gamma, Transactivator HSM-1/2

Molecular Weight: approx. 50 kDa

Gene ID: 4802

NCBI Accession: NP_055038

UniProt: Q13952

Pathways: Regulation of Lipid Metabolism by PPARalpha