NFYC antibody (C-Term)

Catalog No. ABIN2780913

The Rabbit Polyclonal anti-NFYC antibody is suitable to detect NFYC in samples from Human, Mouse, Rat, Dog, Cow, Guinea Pig, Zebrafish (Danio rerio), Rabbit and Horse. It has been validated for WB and IHC.

Quick Overview for NFYC antibody (C-Term) (ABIN2780913)

Target: NFYC (Nuclear Transcription Factor Y, gamma (NFYC))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Zebrafish (Danio rerio), Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NFYC Antibody

Binding Specificity: C-Term

Purpose: NFYC Antibody - C-terminal region

Sequence: GTQVVQGQIQ TLATNAQQIT QTEVQQGQQQ FSQFTDGQQL YQIQQVTMPA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against NFYC. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NFYC

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8106886

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFYC

Target: NFYC (Nuclear Transcription Factor Y, gamma (NFYC))

Alternative Name: NFYC

Background:

Targeted Subunit: gamma

Background Information: NFYC is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. NFYC forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity.The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants. Additional regulation, preliminarily supported by the EST database, may be represented by alternative splicing in this subunit.

Gene Name: Nuclear transcription factor Y, gamma

Alternative Symbols: HSM, CBFC, HAP5, CBF-C, NF-YC, H1TF2A

Protein Name: Nuclear transcription factor Y subunit gamma

Molecular Weight: 37kDa

Gene ID: 4802

NCBI Accession: NP_055038

UniProt: Q13952

Pathways: Regulation of Lipid Metabolism by PPARalpha