TIMP Metallopeptidase Inhibitor 3 (TIMP3) antibody

Antigen: TIMP Metallopeptidase Inhibitor 3 (TIMP3)

Synonyms: SFD, K222, K222TA2, HSMRK222, TIMP3, timp3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN498405

Additional Information

Characteristics: Synonyms: TIMP-3, Tissue inhibitor of metalloproteinases 3, Metalloproteinase inhibitor 3, MIG-5

Alternative name: TIMP3

Gene ID: 7078

Swiss-Prot: P35625

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins thatspecifically inhibit matrix metalloproteinases and regulate extracellular matrix turnoverand tissue remodeling by forming tightly bound inhibitory complexes with the MMPs. Thus,TIMPs maintain the balance between matrix destruction and formation. An imbalancebetween MMPs and the associated TIMPs may play a significant role in the invasivephenotype of malignant tumors. TIMP proteins share several structural features includingsix loops held in place by six disulfide bonds arranged in three knotlike structures. The Nterminal region is necessary for inhibitory activity. The N terminus of each TIMP contains aconsensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leadersequence that is cleaved to produce the mature protein. The C terminal regions aredivergent and enhance the inhibition selectivity and binding efficiency. Although the TIMPproteins share high homology, following secretion they are localized extracellularly eitherin soluble form (TIMP1, TIMP2, and TIMP4) or bound to extracellular matrix components(TIMP3).

Specificity: TIMP-3 pAb detects endogenous levels of TIMP-3 protein. Species: Human, Mouse, Rat. Others not tested.

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1,0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Proteolysis / Ubiquitin, Angiogenesis, Extracellular Matrix, Matrix Metalloproteinases, Apoptosis/Necrosis, Cancer, Inflammation

Restrictions: For Research Use only