PRKAG1 antibody

Catalog No. ABIN498427

This Rabbit Polyclonal antibody specifically detects PRKAG1 in WB and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PRKAG1 antibody (ABIN498427)

Target: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-PRKAG1 Antibody

Specificity: This antibody detects endogenous levels of AMPKgamma1/2/3 protein. (region surrounding Lys78)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PRKAG1

Target: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Alternative Name: AMPK gamma-1 Chain / AMPKg

Background: The 5-AMP activated protein kinase (AMPK), a member of the SNF1 (sucrose non fermentor) kinase family, is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase. AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro.Synonyms: 5'-AMP-activated protein kinase subunit gamma-1/2/3, AMPK gamma-1, AMPK gamma-2, AMPK gamma-3, AMPKG3, AMPKg, H91620p, PRKAG1, PRKAG2, PRKAG3

Molecular Weight: approx. 40,42 kDa

Gene ID: 5571

NCBI Accession: NP_002724

UniProt: P54619

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process, Warburg Effect