ADD2 antibody

Catalog No. ABIN498443

The Rabbit Polyclonal anti-ADD2 antibody is suitable to detect ADD2 in samples from Human, Mouse and Rat. It has been validated for WB and IF.

Quick Overview for ADD2 antibody (ABIN498443)

Target: ADD2 (Adducin 2 (Beta) (ADD2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ADD2 Antibody

Specificity: This antibody detects endogenous levels of Adducin beta protein.(region surrounding Glu497)

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ADD2

Target: ADD2 (Adducin 2 (Beta) (ADD2))

Alternative Name: beta-Adducin (ADD2)

Background: Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta and gamma). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin alpha is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin alpha and Adducin gamma is ubiquitous in contrast to the restricted expression of Adducin beta. Adducin beta is expressed at high levels in brain and hematopoietic tissues, such as bone marrow in humans, and spleen in mice.Synonyms: ADDB, Adducin 2, Erythrocyte adducin subunit beta

Molecular Weight: approx. 81 kDa

Gene ID: 119

NCBI Accession: NP_001608

UniProt: P35612

Pathways: Regulation of Actin Filament Polymerization