IL12B antibody

Catalog No. ABIN498502

This Rabbit Polyclonal antibody specifically detects IL12B in WB. It exhibits reactivity toward Human.

Quick Overview for IL12B antibody (ABIN498502)

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL12B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IL12B Antibody

Specificity: This antibody detects endogenous levels of IL-12B protein. (region surrounding Val277)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: Interleukin-12 beta / IL12B

Background: The interleukins (ILs) are a broad family of well characterized cytokines, primarily of hematopoietic cell origin. As new cytokines are molecularly characterized, they are assigned an IL number to maintain a standard nomenclature. ILs are secreted by immune cells that regulate a wide range of immune system functions. IL-12 is secreted by macrophages and human B-lymphoblastoid cells in response to antigenic stimulation. This IL is responsible for the differentiation of naive CD4+ T cells into type 1 helper T cells that produce interferon-gamma (IFN-gamma). It also activates production of tumor necrosis factor alpha (TNFalpha) from T and natural killer (NK) cells, and it inhibits IL-4 mediated suppression of IFN-gamma. IL-12 also has anti-angiogenic activity since the production of IFN-gamma increases the production of inducible protein-10 (IP-10). IL-12 p40 represents a subunit of IL-12 that is induced by several pathogens. IL-12 p70 is a subunit of IL-12 that is controlled through both the p35 and p40 genes in a cell type-specific fashion.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2

Molecular Weight: approx. 44 kDa

Gene ID: 3593

NCBI Accession: NP_002178

UniProt: P29460

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation