DEFA4 antibody

Catalog No. ABIN498518

The Rabbit Polyclonal anti-DEFA4 antibody is suitable to detect DEFA4 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for DEFA4 antibody (ABIN498518)

Target: DEFA4 (Defensin, alpha 4, Corticostatin (DEFA4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEFA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DEFA4 Antibody

Specificity: This antibody detects endogenous levels of Defensin α4 protein. (region surrounding Asp27)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for DEFA4

Target: DEFA4 (Defensin, alpha 4, Corticostatin (DEFA4))

Alternative Name: Defensin alpha 4

Background: Human neutrophil alpha-defensins (also designated HNPs) are small, cationic, cysteine-rich antimicrobial proteins that play important roles in innate immunity against infectious microbes such as bacteri,a fungi and enveloped viruses. Alpha-defensins are synthesized as inactive precursors and are activated by proteolytic cleavage by MMP-7. Paneth cells in small intestinal crypts secrete the alpha-defensins, which are also termed cryptidins in mice. The cryptidin-4 gene is unique among the Paneth cell alpha defensins in that it is inactive in the duodenum, but expressed at maximal levels in the distal small bowel.Synonyms: DEF4, DEFA4, HNP-4, HP-4, Neutrophil defensin 4

Molecular Weight: approx. 11 kDa

Gene ID: 1669

NCBI Accession: NP_001916

UniProt: P12838