EIF3D antibody

Catalog No. ABIN499071

The Rabbit Polyclonal anti-EIF3D antibody (ABIN499071) specifically detects EIF3D in WB and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for EIF3D antibody (ABIN499071)

Target: EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF3D Antibody

Specificity: This antibody detects endogenous levels of eIF3ζ protein. (region surrounding Arg135)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EIF3D

Target: EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))

Alternative Name: EIF3D / EIF3S7

Background: Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFE, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3.Synonyms: Eukaryotic translation initiation factor 3 subunit 7, Eukaryotic translation initiation factor 3 subunit D, eIF-3-zeta, eIF3 p66

Molecular Weight: approx. 64 kDa

Gene ID: 8664

NCBI Accession: NP_003744

UniProt: O15371

Pathways: Ribonucleoprotein Complex Subunit Organization