EIF3D antibody (Internal Region)

Catalog No. ABIN6258372

Product Details anti-EIF3D Antibody

Target: EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: EIF3D Antibody detects endogenous levels of total EIF3D.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EIF3D, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for EIF3D

Target: EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))

Alternative Name: EIF3D (EIF3D Products)

Synonyms: eif3s7 antibody, eIF3d antibody, DDBDRAFT_0217568 antibody, DDBDRAFT_0219927 antibody, DDB_0217568 antibody, DDB_0219927 antibody, EIF3S7 antibody, An15g03780 antibody, AO090026000811 antibody, eIF3-p66 antibody, eIF3-zeta antibody, 66/67kDa antibody, AA407891 antibody, Eif3s7 antibody, eIF3p66 antibody, wu:fb12a03 antibody, zgc:64097 antibody, eukaryotic translation initiation factor 3 subunit D antibody, eukaryotic translation initiation factor 3 subunit 7 antibody, eukaryotic initiation factor antibody, eIF-3 zeta antibody, eukaryotic translation initiation factor 3, subunit D antibody, translation initiation factor eIF-3 subunit 7 antibody, eukaryotic translation initiation factor 3 subunit D S homeolog antibody, Eukaryotic translation initiation factor 3 subunit D antibody, eif3d antibody, EIF3D antibody, eIF3-S7 antibody, ANI_1_540134 antibody, AOR_1_1456014 antibody, PTRG_07832 antibody, VDBG_06053 antibody, PGTG_10613 antibody, Tsp_00484 antibody, Eif3d antibody, eif3d.S antibody, eif-3.D antibody

Background:

Description: mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a complex required for several steps in the initiation of protein synthesis of a specialized repertoire of mRNAs (PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:18599441, PubMed:25849773). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). In the eIF-3 complex, EIF3D specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs (PubMed:27462815).

Gene: EIF3D

Molecular Weight: 64 kDa

Gene ID: 8664

UniProt: O15371

Pathways: Ribonucleoprotein Complex Subunit Organization