ME1 antibody

Catalog No. ABIN499096

This Rabbit Polyclonal antibody specifically detects ME1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ME1 antibody (ABIN499096)

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ME1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ME1 Antibody

Specificity: This antibody detects endogenous levels of Malic enzyme 1 protein. (region surrounding Tyr500)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for ME1

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Alternative Name: NADP-ME

Background: ME1 (Malic enzyme 1), also known as NADP-ME, MES or HUMNDME, is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family. Expressed ubiquitously with highest expression in liver and white adipose tissue, ME1 functions as an NADP-dependent enzyme that catalyzes the conversion of S-malate and NADP to pyruvate, carbon dioxide and NADPH (a reducing agent that participates in fatty acid biosynthesis). Through its ability to catalyze the reversible oxidative decarboxylation of malate, ME1 links the citric acid and glycolytic cycles. ME1 exists as a homotetramer that uses divalent metal cations, such as magnesium or manganese, as cofactors. The expression of ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet, indicating that ME1 may play an important role as a housekeeping protein within the cell.Synonyms: ME1, NADP-dependent malic enzyme

Molecular Weight: approx. 64 kDa

Gene ID: 4199

NCBI Accession: NP_002386

UniProt: P48163

Pathways: Regulation of Lipid Metabolism by PPARalpha