ME1 antibody (N-Term)

Catalog No. ABIN2777597

The Rabbit Polyclonal anti-ME1 antibody (ABIN2777597) specifically detects ME1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Sheep and Cow samples.

Quick Overview for ME1 antibody (N-Term) (ABIN2777597)

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Sheep, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ME1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ME1 Antibody

Binding Specificity: N-Term

Purpose: ME1 Antibody - N-terminal region

Sequence: QQLNIHGLLP PSFNSQEIQV LRVVKNFEHL NSDFDRYLLL MDLQDRNEKL

Predicted Reactivity: Cow: 92%, Dog: 85%, Horse: 92%, Human: 100%, Mouse: 100%, Pig: 91%, Rabbit: 100%, Rat: 100%, Sheep: 92%

Characteristics: This is a rabbit polyclonal antibody against ME1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ME1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8104821

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ME1

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Alternative Name: ME1

Background:

Background Information: ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Malic enzyme 1, NADP(+)-dependent, cytosolic

Alternative Symbols: MES, HUMNDME

Protein Name: NADP-dependent malic enzyme

Molecular Weight: 64kDa

Gene ID: 4199

NCBI Accession: NP_002386

UniProt: P48163

Pathways: Regulation of Lipid Metabolism by PPARalpha