Tppp antibody (Internal Region)

Catalog No. ABIN499122

Product Details anti-Tppp Antibody

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Tppp antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-DPSKDRAAKR LSLES

Specificity: Recognizes Tubulin polymerization-promoting protein (TPPP).

Cross-Reactivity (Details): Species reactivity (expected):Porcine.
Species reactivity (tested):Human, Mouse and Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the Internal region of the protein sequence according to NP_008961.1. Genename: TPPP

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.001-0.01 μg/mL. Detects a band of approx. 26 kDa in Human Brain (Cerebellum),Mouse and Rat Brain lysates. An additional band of unknown identity was also consistentlyobserved at 16 kDa. This band was successfully blocked by incubation with the immunizingpeptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Tppp

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Alternative Name: TPPP (Tppp Products)

Synonyms: TPPP/p25 antibody, TPPP1 antibody, p24 antibody, p25 antibody, p25alpha antibody, 2900041A09Rik antibody, AI849835 antibody, RGD1310121 antibody, tubulin polymerization promoting protein antibody, TPPP antibody, Tppp antibody

Background: Tubulin Polymerization Promoting Protein (TPPP) promotes in vitro polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.Synonyms: 25 kDa brain-specific protein, TPPP1, Tubulin polymerization-promoting protein

Gene ID: 11076

NCBI Accession: NP_008961

UniProt: O94811