EIF2B4 antibody (Internal Region)

Catalog No. ABIN499136

The Goat Polyclonal anti-EIF2B4 antibody is suitable to detect EIF2B4 in samples from Human, Mouse, Rat, Cow, Rabbit and Dog. It has been validated for WB and EIA.

Quick Overview for EIF2B4 antibody (Internal Region) (ABIN499136)

Target: EIF2B4 (Eukaryotic Translation Initiation Factor 2B, Subunit 4 Delta, 67kDa (EIF2B4))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This EIF2B4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-EIF2B4 Antibody

Binding Specificity: Internal Region

Sequence: C-RKDYGSKVSL FSH

Specificity: Recognizes EIF2B4. This antibody is expected to recognize all reported isoforms (NP_751945.2, NP_001029288.1 and NP_056451.3).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine, Rabbit.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to (NP_751945.2, NP_001029288.1 and NP_056451.3). Genename: EIF2B4

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.3-1 μg/mL. This antibody detects a approx. 60 kDa band in lysates of cell lineK562. An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for EIF2B4

Target: EIF2B4 (Eukaryotic Translation Initiation Factor 2B, Subunit 4 Delta, 67kDa (EIF2B4))

Alternative Name: EIF2B4 / EIF2BD

Background: EIF2B4, also called eIF2B delta is part of the eIF2B complex, which contains 5 subunits. It catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP (referenced from swissprot).Synonyms: Translation initiation factor eIF-2B subunit delta, eIF-2B GDP-GTP exchange factor subunit delta

Gene ID: 8890

NCBI Accession: NP_001029288

UniProt: Q9UI10

Pathways: Methionine Biosynthetic Process