SNAP23 antibody (Internal Region)

Catalog No. ABIN499144

This Goat Polyclonal antibody specifically detects SNAP23 in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat and Cow.

Quick Overview for SNAP23 antibody (Internal Region) (ABIN499144)

Target: SNAP23 (Synaptosomal-Associated Protein, 23kDa (SNAP23))

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This SNAP23 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SNAP23 Antibody

Binding Specificity: Internal Region

Sequence: C-QIKRITDKAD

Specificity: This antibody is expected to recognize both reported isoforms (NP_003816.2 and NP_570710.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_003816.2 and NP_570710.1. Genename: SNAP23

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.1-0.3 μg/mL. This antibody detects a band of approx. 26 kDa Human Placentalysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SNAP23

Target: SNAP23 (Synaptosomal-Associated Protein, 23kDa (SNAP23))

Alternative Name: SNAP23

Background: Synaptosomal-associated proteins (SNAPs) are cytosolic proteins that play a key role in in the process of membrane fusion in intracellular vesicle trafficking. In eukaryotic cells, the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex is critical to membrane docking and fusion and is believed to impart some degree of specificity between vesicle SNARE (v-SNARE) and target organelle SNARE (t-SNARE). In neurons and neuroendocrine cells, the SNARE complex consists of the integral membrane proteins VAMP (vesicle-associated membrane protein), syntaxin and SNAP-25. In non-neuronal tissue, a SNAP-25 homolog, SNAP-23, functionally replaces SNAP-25 in the SNARE complex. Studies show that VAMP, syntaxin and SNAP-23 are required for SNARE function and that this complex exists as a heterotrimer of the three proteins. In insulin signalling pathways, studies suggest that the translocation of GLUT4 in adipocytes requires that functional SNAP-23 be present in SNARE complexes.Synonyms: SNAP-23, Synaptosomal-associated protein 23, Vesicle-membrane fusion protein SNAP-23

Molecular Weight: 23.4kDa (NP_003816.2

Gene ID: 8773

NCBI Accession: NP_003816

UniProt: O00161