SMAD9 antibody (Internal Region)

Catalog No. ABIN499162

This Goat Polyclonal antibody specifically detects SMAD9 in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for SMAD9 antibody (Internal Region) (ABIN499162)

Target: SMAD9 (SMAD Family Member 9 (SMAD9))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This SMAD9 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SMAD9 Antibody

Binding Specificity: Internal Region

Sequence: C-HSEPLMPHNA TYPD

Specificity: This antibody recognizes SMAD9 and is not expected to cross-react with SMAD1 and SMAD5.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human

Purification: Ammonium Sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_005896.1

Application Details

Application Notes: Peptide ELISA: limit dilution 1: 64000. Western blot: 0.3-1.0 μg/mL, approx 50 kDa band observed in Human Skin lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SMAD9

Target: SMAD9 (SMAD Family Member 9 (SMAD9))

Alternative Name: SMAD9

Background: SMAD9 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily.Synonyms: MAD homolog 9, MADH9, Mothers against DPP homolog 9, Mothers against decapentaplegic homolog 9, SMAD 9, SMAD family member 9, SMAD-9

Molecular Weight: 48.6 kDa (NP_005896.1)

Gene ID: 4093

NCBI Accession: NP_001120689

UniProt: O15198