IL-18 antibody (Internal Region)

Catalog No. ABIN499167

Cited in 1 publication. The Goat Polyclonal anti-IL-18 antibody (ABIN499167) specifically detects IL-18 in WB. The antibody is reactive with Human, Rat and Rabbit samples.

Quick Overview for IL-18 antibody (Internal Region) (ABIN499167)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reactivity: Human, Rat, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-18 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IL-18 Antibody

Binding Specificity: Internal Region

Sequence: C-NPPDNIKDTK SDI

Specificity: This antibody detects Interleukin-18.

Cross-Reactivity (Details): Species reactivity (expected):Rabbit, ratSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_001553.1.

Application Details

Application Notes: Peptide ELISA: limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 23 kDa band observed in Human Placenta lysates and inlysates of cell line HeLa (calculated MW of 22.3 kDa according to NP_001553.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

References for anti-IL-18 antibody (ABIN499167)

Rather, Malik, Trikha, Bhat, Dhawan: "Aqueous Terminalia arjuna extract modulates expression of key atherosclerosis-related proteins in a hypercholesterolemic rabbit: A proteomic-based study." in: Proteomics. Clinical applications, Vol. 10, Issue 7, pp. 750-9, (2017) (PubMed).

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: Interleukin-18 / IL18

Background: IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL18 also has other biological properties such as stimulation of Fas ligand expression, and induction of CC and CXC chemokines. IL18 increases production of competent HIV. IL18 is related to the IL1 family. One of the IL18 receptor chains is the IL1 receptor-related protein, a member of the IL 1R family. Like IL1 , IL18 is first synthesized as a leaderless precursor requiring the IL1 converting enzyme (ICE) for cleavage into an active molecule. IL18 may play a role in tissue injury in inflammatory reactions.Synonyms: IGIF, IL-1 gamma, IL-18, IL1 gamma, IL1F4, Iboctadekin, Interferon-gamma-inducing factor, Interleukin-1 gamma

Gene ID: 3606

NCBI Accession: NP_001553

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome