Decorin antibody (Internal Region)

Catalog No. ABIN499170

The Goat Polyclonal anti-Decorin antibody (ABIN499170) specifically detects Decorin in WB. The antibody is reactive with Human, Cow, Pig and Guinea Pig samples.

Quick Overview for Decorin antibody (Internal Region) (ABIN499170)

Target: Decorin (DCN)

Reactivity: Human, Cow, Pig, Guinea Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Decorin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Decorin Antibody

Binding Specificity: Internal Region

Sequence: C-KISRVDAASL KGLNN

Specificity: This antibody detects Decorin (Internal). It is expected to recognize the three reported isoforms a, b and c (NP_001911.1, NP_598011.1, NP_598012.1 resp.). Reported variants NP_598010.1 and NP_001911.1 represent identical protein.

Cross-Reactivity (Details): Species reactivity (expected):Cow, pig, caviaSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_001911.1, NP_598011.1, NP_598012.1

Application Details

Application Notes: Peptide ELISA: limit dilution 1: 8000. Western blot: 0.5-2 μg/mL. Approx 38 kDa band observed in Human Kidney lysates(calculated MW of 39.8 kDa according to NP_001911.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Target Details for Decorin

Target: Decorin (DCN)

Alternative Name: Decorin

Background: Decorin is a member of the small, leucine rich proteoglycan (SLRP) family along with biglycan. Found in the interterritorial region of cartilage, decorin interacts with collagen and other matrix proteins to regulate the cartilaginous matrix. It has been shown to interact with fibronectin, thrombospondin, C1q, epidermal growth factor receptor (EGFR), and TGF beta. It is believed that decorin also plays a role in the cell cycle of chondrocytes.Synonyms: Bone proteoglycan II, DCN, PG-S2, PG40, SLRR1B

Gene ID: 1634

NCBI Accession: NP_001911

UniProt: P07585

Pathways: Glycosaminoglycan Metabolic Process