Decorin antibody (C-Term)

Catalog No. ABIN3044362

Product Details anti-Decorin Antibody

Target: Decorin (DCN)

Binding Specificity: AA 343-359, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Decorin antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Decorin(DCN) detection. Tested with WB in Human,Mouse,Rat.

Sequence: TFRCVYVRSA IQLGNYK

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Decorin(DCN) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: decorin
Protein Name: Decorin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Decorin(343-359aa TFRCVYVRSAIQLGNYK), identical to the related mouse sequence, and different from the related rat sequence by three amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-Decorin antibody (ABIN3044362)

Han, Wang, Jiang, Ma, Tian, Zang, Ma: "An Animal Model of Modic Changes by Embedding Autogenous Nucleus Pulposus inside Subchondral Bone of Lumbar Vertebrae." in: Scientific reports, Vol. 6, pp. 35102, (2016) (PubMed).

Huang, Zou, Shi, Zhang, Pen, Zhang, Gao, Wang: "The effect of electroacupuncture on the extracellular matrix synthesis and degradation in a rabbit model of disc degeneration." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 731395, (2014) (PubMed).

Target Details for Decorin

Target: Decorin (DCN)

Alternative Name: DCN (DCN Products)

Synonyms: CSCD antibody, DSPG2 antibody, PG40 antibody, PGII antibody, PGS2 antibody, SLRR1B antibody, DC antibody, mDcn antibody, cscd antibody, dspg2 antibody, pg40 antibody, pgii antibody, pgs2 antibody, slrr1b antibody, decorin antibody, decorin L homeolog antibody, DCN antibody, Dcn antibody, dcn.L antibody, dcn antibody

Background: Decorin is a proteoglycan on average 90-140 kilodaltons(kD) in size. The human decorin gene spans more than 38 kb and contains 8 exons and very large introns, 2 of which are 5.4 and more than 13.2 kb. And the human decorin gene is mapped to 12q21.3. It belongs to the small leucine-rich proteoglycan(SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan(GAG) chain consisting of either chondroitin sulfate(CS) or dermatan sulfate(DS). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are though to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. And it also may play a role in epithelial/mesenchymal interactions during organ development and shaping. Decorin has been shown to have anti-tumorigenic properties in an experimental murine tumor model and is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for the decorin gene. Mutations in the decorin gene are associated with congenital stromal corneal dystrophy.

Synonyms: Bone proteoglycan II antibody|CSCD antibody|DCN antibody|DCN protein antibody|Decorin antibody|Decorin proteoglycan antibody|Dermatan sulphate proteoglycans II antibody|DKFZp686J19238 antibody|DSPG 2 antibody|DSPG2 antibody|PG 40 antibody|PG II antibody|PG S2 antibody|PG-S2 antibody|PG40 antibody|PGII antibody|PGS 2 antibody|PGS2 antibody|PGS2_HUMAN antibody|Proteoglycan core protein antibody|SLRR1B antibody|Small leucine rich protein 1B antibody

UniProt: P07585

Pathways: Glycosaminoglycan Metabolic Process