AAK1 antibody (N-Term)

Catalog No. ABIN499183

This Rabbit Polyclonal antibody specifically detects AAK1 in WB, IF and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for AAK1 antibody (N-Term) (ABIN499183)

Target: AAK1 (AP2 associated kinase 1 (AAK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-AAK1 Antibody

Binding Specificity: N-Term

Specificity: This antibody recognizes AAK1 near the amino terminus.

Purification: Affinity chromatography purified via peptide column

Immunogen: A 20 amino acid peptide near the amino terminus of the uman Aak1

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Aak1 antibody can be used for detection of Aak1 at 1 - 2 μg/mL. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for AAK1

Target: AAK1 (AP2 associated kinase 1 (AAK1))

Alternative Name: AAK1

Background: AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxf) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist, the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.Synonyms: AP2-associated protein kinase 1, KIAA1048

Gene ID: 22848

NCBI Accession: NP_055726

UniProt: Q2M2I8