You are viewing an incomplete version of our website. Please click to reload the website as full version.

APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (N-Term)

Details for Product anti-APAF1 Antibody No. ABIN499287, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

APAF1, ARK, Apaf-1, Apaf1, CG6829, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dApaf-1, Dark/Hac-1/dApaf1, Dmel\\CG6829, Hac-1, Hac-1/Dark, Hac1, T1, anon- ... show more
APAF1, ARK, Apaf-1, Apaf1, CG6829, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dApaf-1, Dark/Hac-1/dApaf1, Dmel\\CG6829, Hac-1, Hac-1/Dark, Hac1, T1, anon-53Fa, apaf-1, apaf1, arc, ark, dAPAF-1, dApaf-1, dApaf-1/DARK/HAC-1, dApaf1, dArk, dapaf, dapaf-1, dapaf-1L, dapaf-1S, dark, dark/dapaf-1/hac-1, dark/hac-1/dapaf-1, hac-1, hac1, l(2)SH0173, APAF-1, CED4, 6230400I06Rik, Apaf1l, fog, mKIAA0413, zgc:136543 show less
Show less
(47), (44), (18), (13), (13), (8), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(195), (103), (84), (4), (2), (2), (2), (1), (1)
(168), (23), (13), (4)
This APAF1 antibody is un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(153), (63), (60), (44), (29), (22), (16), (10), (8), (4), (4)
Pubmed 4 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Human Apaf1 (N-Terminus) Peptide
Isotype IgG
Specificity Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.
Purification Affinity chromatography purified via peptide column
Alternative Name APAF1 (APAF1 Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues .Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413
Gene ID 317
UniProt O14727
Research Area Cancer, Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis
Application Notes ELISA. Western Blot: Apaf1 antibody can be used for detection of Apaf1 at 1 - 2 μg/mL. Whole celllysate from HeLa cells can be used as positive control and a 115 - 130 kDa band should bedetected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (N-Term) (ABIN499287) Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/ml.
Western Blotting (WB) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (N-Term) (ABIN499287) Western blot analysis of Apaf1 in K562 cell lysate with AP30055PU-N Apaf1 antibody at...
Background publications Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Hu, Benedict, Wu et al.: "Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4386-91, 1998 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

Catalog No. ABIN499287
Plus shipping costs $45.00

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)