PTRH2 antibody (Middle Region)

Catalog No. ABIN499473

This anti-PTRH2 antibody is a Rabbit Polyclonal antibody detecting PTRH2 in WB, IHC (p) and EIA. Suitable for Human and Mouse.

Quick Overview for PTRH2 antibody (Middle Region) (ABIN499473)

Target: PTRH2 (Peptidyl-tRNA Hydrolase 2 (PTRH2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTRH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PTRH2 Antibody

Binding Specificity: Middle Region

Specificity: Bit1 antibody was raised against a 16 amino acid peptide from near the center of human Bit1.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human Bit1 / Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2 (Intermediate Domain) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Bit1 antibody can be used for the detection of Bit1 at 1 - 2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for PTRH2

Target: PTRH2 (Peptidyl-tRNA Hydrolase 2 (PTRH2))

Alternative Name: PTRH2 / BIT1

Background: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (2).Synonyms: CGI-147, PTH2, Peptidyl-tRNA hydrolase 2 mitochondrial

Gene ID: 51651

NCBI Accession: NP_057161

UniProt: Q9Y3E5