Endonuclease G (ENDOG) antibody

Details for Product No. ABIN499796
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Antigen
Synonyms CG8862, Dmel\\CG8862, Tb08.10J17.140, Tb08.10J17.90, TBC1D13, endog, ENDOG, wu:fb79c08, zgc:110020
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(90), (59), (49), (13), (13), (12), (12), (1), (1)
Host
Rabbit
(50), (42)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(82), (38), (11), (10), (8), (7), (2), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN499796
401.50 $
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Immunogen Human EndoG Peptide (AP30316CP-N)
Isotype IgG
Specificity EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.
Purification Affinity chromatography purified via peptide column
Alternative Name Endonuclease G
Background The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).
Alternate names: ENDOG
Gene ID 2021
NCBI Accession NP_004426
UniProt Q14249
Research Area DNA/RNA
Application Notes ELISA. Western 1 to 2 μg/ml. HepG2 and 3T3 cell lysates can be used as positive control and a 35kDa band can be detected. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Hengartner: "Apoptosis. DNA destroyers." in: Nature, Vol. 412, Issue 6842, pp. 27, 29, 2001 (PubMed).

Parrish, Li, Klotz et al.: "Mitochondrial endonuclease G is important for apoptosis in C. elegans." in: Nature, Vol. 412, Issue 6842, pp. 90-4, 2001 (PubMed).

Li, Luo, Wang: "Endonuclease G is an apoptotic DNase when released from mitochondria." in: Nature, Vol. 412, Issue 6842, pp. 95-9, 2001 (PubMed).

Widlak, Li, Wang et al.: "Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 48404-9, 2001 (PubMed).

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