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Details for Product No. ABIN499850

CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (C-Term) antibody

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Antigen
Synonyms
CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, 2310024N18Rik, A430105C05Rik, Cash, ENSMUSG00000072980, Flip, Gm9845, c-Flip, Clarp1, cfl ... show more
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN499850
Quantity 0.1 mg
Price
390.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Human FLIP (C-Terminus) Peptide (AP30343CP-N)
Isotype IgG
Specificity FLIP antibody was raised against a peptide corresponding to amino acids 191 to 209 at C- terminus of human FLIP form2. Antibody recognizes FLIPgamma and FLIPdelta but not FLIPalpha and FLIPbeta.
Purification Affinity chromatography purified via peptide column
Alternative Name CFLAR / Casper / I-FLICE
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively1,2. The human FLIPs were also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH, CLARP and usurpin3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively (8).
Alternate names: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE- like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP
Gene ID 8837
NCBI Accession AAC51623
UniProt O15519
Application Notes ELISA. Western Blot: 1/1000.35 kDa and 25 kDa bands can be detected. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide.
Preservative Sodium azide
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Thome, Schneider, Hofmann et al.: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997 (PubMed).

Shu, Halpin, Goeddel: "Casper is a FADD- and caspase-related inducer of apoptosis." in: Immunity, Vol. 6, Issue 6, pp. 751-63, 1997 (PubMed).

Hu, Vincenz, Ni et al.: "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17255-7, 1997 (PubMed).

Irmler, Thome, Hahne et al.: "Inhibition of death receptor signals by cellular FLIP." in: Nature, Vol. 388, Issue 6638, pp. 190-5, 1997 (PubMed).

Alternatives for antigen "CASP8 and FADD-Like Apoptosis Regulator (CFLAR)", type "Antibodies"
Hosts (141), (30), (2)
Reactivities (157), (77), (53), (25), (25), (25), (12), (1), (1)
Applications (141), (62), (41), (33), (25), (24), (20), (10), (9), (4), (3), (1), (1), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (44), (19), (8), (6), (5), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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