IL-33 antibody (N-Term)

Catalog No. ABIN500012

The Rabbit Polyclonal anti-IL-33 antibody has been validated for WB, IF and EIA. It is suitable to detect IL-33 in samples from Human.

Quick Overview for IL-33 antibody (N-Term) (ABIN500012)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-IL-33 Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to IL-33.

Purification: Affinity chromatography purified via peptide column

Immunogen: IL-33 antibody was raised against a 19 amino acid peptide from near the amino terminus of human IL-33.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 1 μg/mL. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: Interleukin-33 (IL33)

Background: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1a/b, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-kappaB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.Synonyms: C9orf26, IL-1F11, IL-33, IL1F11, Interleukin-1 family member 11, NF-HEV, NFHEV, Nuclear factor from high endothelial venules

Gene ID: 90865

NCBI Accession: NP_254274

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response