IL-33 antibody

Catalog No. ABIN1169172

Cited in 4+ publications. The Mouse Monoclonal anti-IL-33 antibody (Clone IL33305B) (ABIN1169172) specifically detects IL-33 in WB, IHC, ELISA, IP and Func. The antibody is reactive with Human samples.

Quick Overview for IL-33 antibody (ABIN1169172)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Functional Studies (Func)

Clone: IL33305B

Product Details anti-IL-33 Antibody

Purpose: anti-IL-33 (human), mAb (IL33305B)

Specificity: Recognizes human IL-33. Does not cross-react with mouse IL-33.

No Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Does not cross-react with mouse IL-33.

Characteristics: Monoclonal Antibody. Recognizes human IL-33. Does not cross-react with mouse IL-33. Isotype: Mouse IgG2akappa. Clone: IL33305B. Applications: ELISA, FUNC (Blocking), IHC, IP, WB. Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. Interleukin-33 (IL-33, HF-NEV, IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30 kDa human IL33 is converted by CASP1 to a 18 kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Sterility: 0.2 μm filtered

Immunogen: Recombinant human IL-33.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.2μm-filtered solution in PBS, pH 7.4.

Preservative: Without preservative

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-IL-33 antibody (ABIN1169172)

Pace, Di Sano, Sciarrino, Scafidi, Ferraro, Chiappara, Siena, Gangemi, Vitulo, Giarratano, Gjomarkaj: "Cigarette smoke alters IL-33 expression and release in airway epithelial cells." in: Biochimica et biophysica acta, Vol. 1842, Issue 9, pp. 1630-7, (2014) (PubMed).

Palmer, Talabot-Ayer, Lamacchia, Toy, Seemayer, Viatte, Finckh, Smith, Gabay: "Inhibition of interleukin-33 signaling attenuates the severity of experimental arthritis." in: Arthritis and rheumatism, Vol. 60, Issue 3, pp. 738-49, (2009) (PubMed).

Chapuis, Hot, Hansmannel, Kerdraon, Ferreira, Hubans, Maurage, Huot, Bensemain, Laumet, Ayral, Fievet, Hauw, DeKosky, Lemoine, Iwatsubo, Wavrant-Devrièze, Dartigues, Tzourio, Buée, Pasquier, Berr et al.: "Transcriptomic and genetic studies identify IL-33 as a candidate gene for Alzheimer's disease. ..." in: Molecular psychiatry, Vol. 14, Issue 11, pp. 1004-16, (2009) (PubMed).

Talabot-Ayer, Lamacchia, Gabay, Palmer: "Interleukin-33 is biologically active independently of caspase-1 cleavage." in: The Journal of biological chemistry, Vol. 284, Issue 29, pp. 19420-6, (2009) (PubMed).

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL-33

Background:

Alternate Names/Synonyms: Interleukin-33, IL-1F11, NF-HEV

Product Description: Interleukin-33 (IL-33, HF-NEV, IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30 kDa human IL33 is converted by CASP1 to a 18 kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response