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Interleukin-1 Receptor-Associated Kinase 2 (IRAK2) (C-Term) antibody

Details for Product No. ABIN500042, Supplier: Log in to see
Antigen
  • IRAK-2
  • 6330415L08Rik
  • AI649099
Alternatives
anti-Human Interleukin-1 Receptor-Associated Kinase 2 antibody for Immunofluorescence
Epitope
C-Term
72
20
15
14
9
8
7
6
5
5
5
3
3
2
2
2
2
1
1
Reactivity
Human
177
38
22
Host
Rabbit
170
27
Clonality
Polyclonal
Conjugate
Un-conjugated
10
10
7
6
6
6
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
172
108
35
31
22
13
11
8
5
4
1
Supplier
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Immunogen Human IRAK2 (C-Terminus) Peptide
Isotype IgG
Specificity IRAK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK2.
Purification Affinity chromatography purified via peptide column
Alternative Name IRAK2 (IRAK2 Antibody Abstract)
Background The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappaB , which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (2). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappaB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kappaB activation (3,4). Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory responses to cytokine IL-1 and LPS. IRAK2 is expressed in a variety of human tissues.Synonyms: IRAK-2, Interleukin-1 receptor-associated kinase-like 2
Gene ID 108960
UniProt Q8CFA1
Research Area Immunology, Innate Immunity, Cytokines, Receptors, Inflammation
Pathways TLR Signaling
Application Notes ELISA. Western Blot: 17500 to 1/1000. A 65 kDa band can be detected. Anti-IRAK2 has no crossresponse to IRAK. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunofluorescence (IF) image for anti-Interleukin-1 Receptor-Associated Kinase 2 (IRAK2) (C-Term) antibody (ABIN500042) Immunofluorescence of IRAK2 in HeLa cells with AP30441PU-N IRAK antibody at 10 μg/ml.
Western Blotting (WB) image for anti-Interleukin-1 Receptor-Associated Kinase 2 (IRAK2) (C-Term) antibody (ABIN500042) Western blot analysis of IRAK2 in K562 whole cell lysate with AP30441PU-N IRAK2 antib...
Background publications Yang, Mark, Gurney et al.: "Signaling events induced by lipopolysaccharide-activated toll-like receptor 2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 2, pp. 639-43, 1999 (PubMed).

Zhang, Kirschning, Mancinelli et al.: "Bacterial lipopolysaccharide activates nuclear factor-kappaB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes." in: The Journal of biological chemistry, Vol. 274, Issue 12, pp. 7611-4, 1999 (PubMed).

Muzio, Ni, Feng et al.: "IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1612-5, 1997 (PubMed).

Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996 (PubMed).