KDM3B antibody (Center)

Catalog No. ABIN500076

This anti-KDM3B antibody is a Rabbit Polyclonal antibody detecting KDM3B in WB, IHC (p) and EIA. Suitable for Human, Mouse and Rat.

Quick Overview for KDM3B antibody (Center) (ABIN500076)

Target: KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM3B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-KDM3B Antibody

Binding Specificity: Center

Specificity: This antibody detects JMJD1B. It will not cross-react with JMJD1A or JMJD1C.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: JMJD1B antibody was raised against a 20 amino acid peptide from near the center of human JMJD1B.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for KDM3B

Target: KDM3B (Lysine (K)-Specific Demethylase 3B (KDM3B))

Alternative Name: JMJD1B / KDM3B

Background: The jumonji domain containing 1B protein (JMJD1B) was originally discovered as a candidate for the myeloid leukemia tumor suppressor gene. Besides CD34+ cells and acute myeloid leukemia (AML) cell lines, JMJD1B mRNA is highly expressed in liver, heart, kidney, skeletal muscle, and placenta tissues. The JMJD1B gene is localized to a region of chromosome 5q31, which is frequently deleted in myeloid leukemias and myelodysplasias and expression of JMJD1B in a del(5q) cell line results in suppression of clonogenic growth suggesting that JMJD1B may function as a tumor suppressor. In contrast, JMJD1B gene copy number and mRNA expression level was increased in several non-small cell lung cancers indicating that the role of JMJD1B in cancer formation and progression is more complex than originally postulated. At least three isoforms of JMJD1B are known to exist.Synonyms: 5qNCA, C5orf7, JHDM2B, JmjC domain-containing histone demethylation protein 2B, Jumonji domain-containing protein 1B, KIAA1082, Lysine-specific demethylase 3B, Nuclear protein 5qNCA

Gene ID: 51780

NCBI Accession: NP_057688

UniProt: Q7LBC6

Pathways: Warburg Effect