ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3) (N-Term) antibody

Details for Product No. ABIN500425
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Antigen
Synonyms TMEM142C, BC061259, Tmem142c, 9930124N15, RGD1306538, ORAI3
Epitope
N-Term
(23), (22), (7)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(58), (24), (21)
Host
Rabbit
(39), (19)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(58), (41), (21), (8), (6), (6), (2), (1)
Pubmed 4 references available
Catalog no. ABIN500425
Quantity 0.1 mg
Price
401.50 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen ORAI3 antibody was raised against a 15 amino acid peptide from near the amino terminus of human ORAI3.
Isotype IgG
Specificity This antibody detects ORAI3 at N-term. It is predicted to have no cross-reactivity to ORAI1 or ORAI2. Species Reactivity: Tested: Human, mouse, rat
Purification Peptide affinity chromatography
Alternative Name ORAI3
Background Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter.
Alternate names: ORAI-3, TMEM142C, Transmembrane Protein 142C
Gene ID 93129
NCBI Accession Q9BRQ5
UniProt Q9BRQ5
Application Notes ELISA. Western blot: 1 – 2 μg/ml. Immunoflourescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Background publications Lewis: "Calcium signaling mechanisms in T lymphocytes." in: Annual review of immunology, Vol. 19, pp. 497-521, 2001 (PubMed).

Feske, Gwack, Prakriya et al.: "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function." in: Nature, Vol. 441, Issue 7090, pp. 179-85, 2006 (PubMed).

Mercer, Dehaven, Smyth et al.: "Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1." in: The Journal of biological chemistry, Vol. 281, Issue 34, pp. 24979-90, 2006 (PubMed).

Soboloff, Spassova, Dziadek et al.: "Calcium signals mediated by STIM and Orai proteins--a new paradigm in inter-organelle communication." in: Biochimica et biophysica acta, Vol. 1763, Issue 11, pp. 1161-8, 2006 (PubMed).

Hosts (39), (19)
Reactivities (58), (24), (21)
Applications (58), (41), (21), (8), (6), (6), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1)
Epitopes (23), (22), (7)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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