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ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3) (N-Term) antibody

Details for Product No. ABIN500425, Supplier: Log in to see
Antigen
  • ORAI3
  • TMEM142C
  • 9930124N15
  • BC061259
  • Tmem142c
  • RGD1306538
Epitope
N-Term
35
26
18
Reactivity
Human, Mouse (Murine), Rat (Rattus)
69
20
18
Host
Rabbit
55
14
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
67
42
14
10
9
4
4
3
3
1
Supplier
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Immunogen ORAI3 antibody was raised against a 15 amino acid peptide from near the amino terminus of human ORAI3.
Isotype IgG
Specificity This antibody detects ORAI3 at N-term. It is predicted to have no cross-reactivity to ORAI1 or ORAI2.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Alternative Name ORAI3 (ORAI3 Antibody Abstract)
Background Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter.Synonyms: ORAI-3, TMEM142C, Transmembrane Protein 142C
Gene ID 93129
UniProt Q9BRQ5
Pathways
Application Notes ELISA. Western blot: 1 - 2 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3) (N-Term) antibody (ABIN500425) Immunocytochemistry of ORAI3 in A20 cells with this product at 10 μg/ml.
Western Blotting (WB) image for anti-ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3) (N-Term) antibody (ABIN500425) Western blot analysis of ORAI3 in A20 cell lysate with this product at (A) 1, (B) 2 a...
Background publications Soboloff, Spassova, Dziadek et al.: "Calcium signals mediated by STIM and Orai proteins--a new paradigm in inter-organelle communication." in: Biochimica et biophysica acta, Vol. 1763, Issue 11, pp. 1161-8, 2006 (PubMed).

Mercer, Dehaven, Smyth et al.: "Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1." in: The Journal of biological chemistry, Vol. 281, Issue 34, pp. 24979-90, 2006 (PubMed).

Feske, Gwack, Prakriya et al.: "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function." in: Nature, Vol. 441, Issue 7090, pp. 179-85, 2006 (PubMed).

Lewis: "Calcium signaling mechanisms in T lymphocytes." in: Annual review of immunology, Vol. 19, pp. 497-521, 2001 (PubMed).