ORAI3 antibody (C-Term)

Catalog No. ABIN492535

Product Details anti-ORAI3 Antibody

Target: ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))

Binding Specificity: C-Term

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ORAI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: Recognizes ORAI3. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE, these may represent post-translationally modified ORAI3.

Characteristics: Synonyms: TMEM142C, ORAI-3, Transmembrane Protein 142C

Purification: Immunoaffinity Chromotography.

Immunogen: A 19 amino acid peptide from near the carboxy terminus of Human ORAI3

Clone: 2H2G9

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: ORAI3 antibody can be used for detection of ORAI3 at 2-4 μg/mL. Immunohistochemistry: 2.5 μg/mL. Positive Control: Rat spleen tissue lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ORAI3

Target: ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))

Alternative Name: ORAI3 (ORAI3 Products)

Synonyms: ORAI3 antibody, TMEM142C antibody, 9930124N15 antibody, BC061259 antibody, Tmem142c antibody, RGD1306538 antibody, ORAI calcium release-activated calcium modulator 3 antibody, ORAI3 antibody, Orai3 antibody

Background: Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2 (5). Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter.Synonyms: ORAI-3, TMEM142C, Transmembrane Protein 142C

Gene ID: 93129

UniProt: Q9BRQ5