POLR3F antibody (N-Term)

Catalog No. ABIN500523

The Rabbit Polyclonal anti-POLR3F antibody (ABIN500523) specifically detects POLR3F in WB, IHC (p) and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for POLR3F antibody (N-Term) (ABIN500523)

Target: POLR3F (Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-POLR3F Antibody

Binding Specificity: N-Term

Specificity: This antibody detects POLR3F.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: POLR3F antibody was raised against a 21 amino acid peptide from near the amino terminus of human POLR3F.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for POLR3F

Target: POLR3F (Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F))

Alternative Name: POLR3F

Background: The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.Synonyms: DNA-directed RNA polymerase III subunit F, DNA-directed RNA polymerase III subunit RPC6, RNA polymerase III 39 kDa subunit, RNA polymerase III subunit C6, RPC39, RPC6

Gene ID: 10621

UniProt: Q9H1D9