F-Box and Leucine-Rich Repeat Protein 20 (FBXL20) (N-Term) antibody

Antigen: F-Box and Leucine-Rich Repeat Protein 20 (FBXL20)

Synonyms: SCR, Fbl2, Fbl20, FLJ21037, MGC15482, SCRAPPER, fbl20, MGC81000, scrapper

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN500692

Additional Information

Characteristics: Synonyms: FBL2, F-box/LRR-repeat protein 20, F-box and leucine-rich repeat protein 20,F-box/LRR-repeat protein 2-like

Alternative name: FBXL20

Gene ID: 84961

Swiss-Prot: Q96IG2

Immunogen: FBXL20 antibody was raised against a 18 amino acid peptide near the amino terminus ofthe human FBXL20.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG  (Matching secondary antibodies)

Description: Members of the F-box protein family, such as Scrapper, are characterized by anapproximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, andF-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in thebrain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals which suggests thatit might function as an important membrane-localized E3 ligase in various species. Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradationand the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptictransmission. This identifies protein degradation as a mechanism for holding synapticcommunication in check.

Specificity: This antibody detects FBXL20 at N-term. Species Reactivity: Tested: Human, mouse, ratAdd. Information: Blocking peptide available: ABIN500691

Application Details

Application Notes: ELISA. Western blot: 0.5 – 1 μg/ml. Immunohistochemistry on paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Aff - Purified

Buffer: PBS containing 0.02% sodium azide

Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeatedfreezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only