RANKL antibody (Center)

Catalog No. ABIN500805

Cited in 2+ publications. The Rabbit Polyclonal anti-RANKL antibody (ABIN500805) specifically detects RANKL in WB, IHC (p) and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RANKL antibody (Center) (ABIN500805)

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RANKL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-RANKL Antibody

Binding Specificity: Center

Specificity: This antibody detects CD254 / RANKL. It will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Immunoaffinity Chromatography

Immunogen: sRANK-L antibody was raised against a 14 amino acid peptide from near the center of Human sRANK-L.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.25 - 0.5 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-RANKL antibody (ABIN500805)

Kirschneck, Küchler, Wolf, Spanier, Proff, Schröder: "Effects of the Highly COX-2-Selective Analgesic NSAID Etoricoxib on Human Periodontal Ligament Fibroblasts during Compressive Orthodontic Mechanical Strain." in: Mediators of inflammation, Vol. 2019, pp. 2514956, (2019) (PubMed).

Ullrich, Schröder, Jantsch, Spanier, Proff, Kirschneck: "The role of mechanotransduction versus hypoxia during simulated orthodontic compressive strain-an in vitro study of human periodontal ligament fibroblasts." in: International journal of oral science, Vol. 11, Issue 4, pp. 33, (2019) (PubMed).

Target Details for RANKL

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: CD254 / RANKL

Background: The receptor activator of NF-kappaB ligand (RANK-L) is a recently discovered member of the TNF-ligand family involved in the regulation of the T cell-dependent immune response, lymph node organogenesis and bone formation. RANK-L exists as both a normal, transmembrane form and a truncated, soluble form (sRANK-L), both of which can stimulate the receptor. Activation of T cells, such as by treatment with interleukin-7, induces RANK-L production and leads to an increase of osteoclast formation and bone loss. Finally, sRANK-L can activate the antiapoptotic kinase Akt through a signaling complex involving Src kinase and TRAF6, suggesting sRANK-L may also play a role in regulating apoptosis.Synonyms: ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK Ligand, RANKL, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11

Gene ID: 8600

NCBI Accession: NP_003692

Pathways: NF-kappaB Signaling