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Thioesterase Superfamily Member 4 (THEM4) (Center) antibody

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Synonyms THEM4, CTMP, 2700077M13Rik, 4921507I02Rik, RGD1304723
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(3), (2), (1), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(24), (11), (11)
(22), (2)
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(23), (16), (14), (7), (4), (1), (1)
Pubmed 3 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen THEM4 antibody was raised against an 18 amino acid peptide near the center of human THEM4.
Isotype IgG
Specificity This antibody detects THEM4 / CTMP.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chropmatography
Alternative Name THEM4 / CTMP (THEM4 Antibody Abstract)
Background THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program. The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.Synonyms: Carboxyl-terminal modulator protein, Thioesterase superfamily member 4
Gene ID 117145
NCBI Accession NP_444283
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Thioesterase Superfamily Member 4 (THEM4) (Center) antibody (ABIN500905) Immunohistochemistry of THEM4 in human liver tissue with this product at 2.5 μg/ml.
Western Blotting (WB) image for anti-Thioesterase Superfamily Member 4 (THEM4) (Center) antibody (ABIN500905) Western blot analysis of THEM4 in human liver tissue lysate with this product at (A) ...
Background publications Zhao, Martin, Bisoffi et al.: "The Akt C-terminal modulator protein is an acyl-CoA thioesterase of the Hotdog-Fold family." in: Biochemistry, Vol. 48, Issue 24, pp. 5507-9, 2009 (PubMed).

Parcellier, Tintignac, Zhuravleva et al.: "Carboxy-Terminal Modulator Protein (CTMP) is a mitochondrial protein that sensitizes cells to apoptosis." in: Cellular signalling, Vol. 21, Issue 4, pp. 639-50, 2009 (PubMed).

Maira, Galetic, Brazil et al.: "Carboxyl-terminal modulator protein (CTMP), a negative regulator of PKB/Akt and v-Akt at the plasma membrane." in: Science (New York, N.Y.), Vol. 294, Issue 5541, pp. 374-80, 2001 (PubMed).

Catalog No. ABIN500905
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