THEM4 antibody (Center)

Catalog No. ABIN500905

Product Details anti-THEM4 Antibody

Target: THEM4 (Thioesterase Superfamily Member 4 (THEM4))

Binding Specificity: Center

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THEM4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects THEM4 / CTMP.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chropmatography

Immunogen: THEM4 antibody was raised against an 18 amino acid peptide near the center of human THEM4.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for THEM4

Target: THEM4 (Thioesterase Superfamily Member 4 (THEM4))

Alternative Name: THEM4 / CTMP (THEM4 Products)

Synonyms: THEM4 antibody, CTMP antibody, 2700077M13Rik antibody, 4921507I02Rik antibody, RGD1304723 antibody, thioesterase superfamily member 4 antibody, thioesterase superfamily member 4 L homeolog antibody, THEM4 antibody, them4 antibody, Them4 antibody, them4.L antibody

Background: THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program. The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.Synonyms: Carboxyl-terminal modulator protein, Thioesterase superfamily member 4

Gene ID: 117145

NCBI Accession: NP_444283

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway