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XiAP Associated Factor 1 (XAF1) (C-Term) antibody

Details for Product No. ABIN501126, Supplier: Login to see
Antigen
  • BIRC4BP
  • HSXIAPAF1
  • XIAPAF1
  • Fbxo39
Epitope
C-Term
23
16
16
10
7
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
66
32
7
Host
Rabbit
80
5
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
78
28
18
16
4
4
2
1
Supplier
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Immunogen XAF-1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1.
Isotype IgG
Specificity This antibody detects XAF-1.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse
Purification Affinity chromatography
Alternative Name XAF1 (XAF1 Antibody Abstract)
Background XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus (1,2). Overexpression of XAF-1 results in the neutralization of XIAP\'s ability to inhibit cell death (1). XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines (3). In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines (2,3). Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs (4).Synonyms: BIRC4-binding protein, BIRC4BP, XIAP-associated factor 1, XIAPAF1
Gene ID 54739
NCBI Accession NP_059993
Pathways
Application Notes ELISA. Immunohistochemistry on paraffin sections. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-XiAP Associated Factor 1 (XAF1) (C-Term) antibody (ABIN501126) Immunohistochemical staining of human spleen tissue using AP30988PU-N at 2 μg/ml.
Western Blotting (WB) image for anti-XiAP Associated Factor 1 (XAF1) (C-Term) antibody (ABIN501126) Western blot analysis of XAF-1 in human spleen lysate with this product at 0.5 (lane ...
Background publications Liston, Fong, Kelly et al.: "Identification of XAF1 as an antagonist of XIAP anti-Caspase activity." in: Nature cell biology, Vol. 3, Issue 2, pp. 128-33, 2001 (PubMed).