Zinc Finger, BED-Type Containing 1 (ZBED1) antibody

Antigen: Zinc Finger, BED-Type Containing 1 (ZBED1)

Synonyms: ALTE, DREF, TRAMP, hDREF, KIAA0785, ZBED1, ZBED1P

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN501137

Additional Information

Characteristics: Synonyms: ALTE, DREF, KIAA0785, Zinc finger BED domain-containing protein 1, dREF homolog,Putative Ac-like transposable element

Alternative name: ZBED1

Gene ID: 9189

Swiss-Prot: O96006

Immunogen: ZBED1 antibody was raised against a 15 amino acid peptide from near the center of humanZBED1.

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG  (Matching secondary antibodies)

Description: ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, atranscriptional regulatory factor required for expression of genes involved in DNAreplication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNAresulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells,suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters, anti-ZBED1 RNAialso resulted in decreased RP gene expression. Immunoprecipitation analyses have shownthat ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and thisself-association is required for nuclear localization and DNA binding.

Specificity: This antibody detects ZBED1. The antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3. Species Reactivity: Tested: Human, mouseAdd. Information: Blocking peptide available: ABIN501136

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Aff - Purified

Buffer: PBS containing 0.02% sodium azide

Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeatedfreezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only