KCNMB4 antibody (Middle Region)

Catalog No. ABIN501386

The Rabbit Polyclonal anti-KCNMB4 antibody is suitable to detect KCNMB4 in samples from Human, Mouse, Rat, Sheep, Cow, Guinea Pig and Rabbit. It has been validated for WB.

Quick Overview for KCNMB4 antibody (Middle Region) (ABIN501386)

Target: KCNMB4 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, beta Member 4 (KCNMB4))

Reactivity: Human, Mouse, Rat, Sheep, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNMB4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KCNMB4 Antibody

Binding Specificity: Middle Region

Sequence: TCGADCRGTS QYPCVQVYVN NSESNSRALL HSDEHQLLTN PKCSYIPPCK

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against KCNMB4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human KCNMB4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 210 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for KCNMB4

Target: KCNMB4 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, beta Member 4 (KCNMB4))

Alternative Name: KCNMB4

Background: KCNMB4 is the regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. KCNMB4 modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. KCNMB4 decreases the gating kinetics and calcium sensitivity of the KCNMA1 channel, but with fast deactivation kinetics. KCNMB4 may decrease KCNMA1 channel openings at low calcium concentrations but increases channel openings at high calcium concentrations. KCNMB4 makes KCNMA1 channel resistant to 100 nM charybdotoxin (CTX) toxin concentrations.MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which slows activation kinetics, leads to steeper calcium sensitivity, and shifts the voltage range of current activation to more negative potentials than does the beta 1 subunit.
Alias Symbols: -
Protein Size: 210

Molecular Weight: 24 kDa

Gene ID: 27345

NCBI Accession: NM_014505, NP_055320

UniProt: Q86W47