TRPV4 antibody (Middle Region)

Catalog No. ABIN501411

Cited in 1 publication. The Rabbit Polyclonal anti-TRPV4 antibody (ABIN501411) specifically detects TRPV4 in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Cow, Pig, Dog, Rabbit and Guinea Pig samples.

Quick Overview for TRPV4 antibody (Middle Region) (ABIN501411)

Target: TRPV4 (Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4))

Reactivity: Human, Rat, Mouse, Cow, Pig, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPV4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TRPV4 Antibody

Binding Specificity: Middle Region

Purpose: TRPV4 Antibody - middle region

Sequence: RVDEVNWSHW NQNLGIINED PGKNETYQYY GFSHTVGRLR RDRWSSVVPR

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 86%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against TRPV4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRPV4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP35416-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-TRPV4 antibody (ABIN501411)

Sampat, Dermksian, Oungoulian, Winchester, Bulinski, Ateshian, Hung: "Applied osmotic loading for promoting development of engineered cartilage." in: Journal of biomechanics, Vol. 46, Issue 15, pp. 2674-81, (2013) (PubMed).

Target Details for TRPV4

Target: TRPV4 (Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4))

Alternative Name: TRPV4

Background:

Background Information: TRPV4 is a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. TRPV4 is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure.This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: Transient receptor potential cation channel, subfamily V, member 4

Alternative Symbols: SMAL, VRL2, BCYM3, CMT2C, SPSMA, TRP12, VROAC, HMSN2C, OTRPC4, SSQTL1

Protein Name: Transient receptor potential cation channel subfamily V member 4

Molecular Weight: 98 kDa

Gene ID: 59341

NCBI Accession: NP_671737

UniProt: Q9HBA0

Pathways: Hormone Transport, Cell-Cell Junction Organization