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Antibodies
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anti-Engrailed Homeobox 1 (EN1) antibody
Engrailed Homeobox 1 (EN1) antibody
| Antigen |
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| Synonyms |
En-1, Mo-en.1, EN1 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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 Alternatives Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish
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Conjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
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Application
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| Catalog no. |
ABIN501632 |
| Quantity |
50 µg (Variants) |
| Price |
317.90 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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Engrailed-1 / EN1
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Gene ID
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EN1
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UniProt
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Q05925
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Immunogen
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The immunogen for anti-EN1 antibody: synthetic peptide directed towards the middle region of human EN1
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Sequence
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LNESQIKIWFQNKRAKIKKATGIKNGLALHLMAQGLYNHS TTTVQDKDES
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Cross-Reactivity
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Chicken, Human, Mouse (Murine), Rat (Rattus)
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Format
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Lyophilized
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Reconstitution
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Add 50 µl of distilled water. Final anti-EN1 antibody concentration is 1 mg/ml.
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Description
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Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Differe
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Characteristics
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Antigen Size: 392 amino acids
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Molecular Weight
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40kDa
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Application Notes
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WB Suggested Anti-EN1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: THP-1 cell lysate.
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Purification
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Affinity Purified
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Buffer
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PBS
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Storage (Long Term)
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For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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Storage (Short Term)
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Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
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Restrictions
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For Research Use only
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Product
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Atit, Sgaier, Mohamed et al.: "Beta-catenin activation is necessary and sufficient to specify the dorsal dermal fate in the mouse." in: Developmental biology, Vol. 296, Issue 1, pp. 164-76, 2006 (PubMed).
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Alternatives for antigen "Engrailed Homeobox 1 (EN1)", type "Antibodies"
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Hosts
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Rabbit (28), Mouse (4)
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Reactivities
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Human (31), Mouse (Murine) (19), Rat (Rattus) (18), Chicken (1), Xenopus laevis (1), Zebrafish (1)
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Applications
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Western Blotting (WB) (17), Immunofluorescence (IF) (15), ELISA (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
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Epitopes
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N-Term (3), Center (2), Internal Region (2), C-Term (1)
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2013 BioMed Central Research Awards
