GAPDH antibody (N-Term)

Catalog No. ABIN501931

This Rabbit Polyclonal antibody specifically detects GAPDH in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Dog, Cow, Sheep, Goat, Guinea Pig and Horse. It has been mentioned in 2+ publications

Quick Overview for GAPDH antibody (N-Term) (ABIN501931)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Sheep, Goat, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GAPDH Antibody

Binding Specificity: N-Term

Purpose: GAPDH Antibody - N-terminal region

Sequence: IDLNYMVYMF QYDSTHGKFH GTVKAENGKL VINGNPITIF QERDPSKIKW

Predicted Reactivity: Cow: 93%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against GAPDH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GAPDH

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40175-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-GAPDH antibody (ABIN501931)

Kalinec, Thein, Parsa, Yorgason, Luxford, Urrutia, Kalinec: "Acetaminophen and NAPQI are toxic to auditory cells via oxidative and endoplasmic reticulum stress-dependent pathways." in: Hearing research, Vol. 313, pp. 26-37, (2014) (PubMed).

Fu, Mukhamedova, Ip, DSouza, Henley, DiTommaso, Kesani, Ditiatkovski, Jones, Lane, Jennings, Smyth, Kile, Sviridov: "ABCA12 regulates ABCA1-dependent cholesterol efflux from macrophages and the development of atherosclerosis." in: Cell metabolism, Vol. 18, Issue 2, pp. 225-38, (2013) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background:

Background Information: GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Glyceraldehyde-3-phosphate dehydrogenase

Alternative Symbols: G3PD, GAPD, HEL-S-162eP

Protein Name: Glyceraldehyde-3-phosphate dehydrogenase RuleBase RU003389

Molecular Weight: 36 kDa

Gene ID: 2597

NCBI Accession: NP_002037

UniProt: Q2TSD0