NR4A1 antibody (Middle Region)

Catalog No. ABIN502649

There is 1 publication for this product available. The Rabbit Polyclonal anti-NR4A1 antibody is suitable to detect NR4A1 in samples from Human, Rat, Mouse, Dog, Cow, Horse, Rabbit and Guinea Pig. It has been validated for WB and IHC.

Quick Overview for NR4A1 antibody (Middle Region) (ABIN502649)

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR4A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NR4A1 Antibody

Binding Specificity: Middle Region

Sequence: FQKCLAVGMV KEVVRTDSLK GRRGRLPSKP KQPPDASPAN LLTSLVRAHL

Predicted Reactivity: Cow: 85%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NR4A1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NR4A1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 598 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NR4A1 antibody (ABIN502649)

Nowakowski, Pollen, Sandoval-Espinosa, Kriegstein: "Transformation of the Radial Glia Scaffold Demarcates Two Stages of Human Cerebral Cortex Development." in: Neuron, Vol. 91, Issue 6, pp. 1219-1227, (2016) (PubMed).

Target Details for NR4A1

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Alternative Name: NR4A1

Background: NR4A1 encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Alias Symbols: GFRP1, HMR, MGC9485, N10, NAK-1, NGFIB, NP10, NUR77, TR3
Protein Interaction Partner: KRTAP10-5, KRTAP10-1, LONRF1, PGBD1, BCL2L10, CDKN2D, BCL2A1, BCL2, TUBB4A, VASP, ANKIB1, CUTA, NFKB1, SMAD3, LSS, HADHA, GLUD1, GK, CPT1A, SERPING1, ABCC6, ABCC13, SOCS4, ABCG8, DIABLO, HCVgp1, ABCA5, CHEK2, SUGT1, PRDX4, PRDX6, CHD1L, ABCB11, ARHGEF5, U
Protein Size: 598

Molecular Weight: 64 kDa

Gene ID: 3164

NCBI Accession: NM_002135, NP_002126

UniProt: P22736

Pathways: Fc-epsilon Receptor Signaling Pathway, Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Steroid Hormone Mediated Signaling Pathway